Immunoenzymatic labelling pattern of dihydrofolate reductase using alkaline-phosphatase/anti-alkaline phosphatase(APAAP) method in haemic cells.
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چکیده
منابع مشابه
Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.
The use of alkaline phosphatase in an immunoenzymatic procedure for the localisation of antigens in paraffin sections or cell smears is described. The results of this method, when applied to the detection of immunoglobulins, lysozyme, or lactoferrin, were comparable in intensity and clarity to those obtained with the PAP immunoperoxidase procedure. Furthermore, double immunoenzymatic labelling ...
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proliferative disorders. It is necessary to accurately identify the megakaryocytic cells in order to diagnose megakaryocytic leukemia and to assess the significance of the pres ence of such cells in the circulating blood . At present, megakaryocytes and their precursors can be best identified either by ultrastructural study of platelet peroxidase or by immunocytochemical studies using monoclona...
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We used two different methods to study the expression pattern of alkaline phosphatase (alp) in Dictyostelium. In situ staining of the endogenous enzyme activity at different stages of development showed that the enzyme was active early in the aggregation stage and localized to the area where the tip of the first finger was initiated. The activity was localized to the anterior region of developi...
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Alkaline phosphatase is an enzyme commonly expressed in almost all living organisms. In humans and other mammals, determinations of the expression and activity of alkaline phosphatase have frequently been used for cell determination in developmental studies and/or within clinical trials. Alkaline phosphatase also seems to be one of the key markers in the identification of pluripotent embryonic ...
متن کاملAlkaline phosphatase for immunocytochemical labelling: problems with endogenous enzyme activity.
Alkaline phosphatase may be used as a label for immunocytochemistry and can be demonstrated in tissue sections using the single step naphthol phosphate method. Endogenous enzyme activity may not be destroyed by fixation in formalin, formol alcohol, Carnoy's or Baker's solutions and should be inhibited before results are assessed. Either Bouin's solution or periodic acid followed by potassium bo...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1988
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.21.499